LOCUS       pLV-[hsa-miR-517a]        9139 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7060..7920
                     /label=Amp(R)
     promoter        8121..8661
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5665..5899
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6474..6929)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5971..6101
                     /label=SV40 pA
     misc_feature    6102..6315
                     /label=SV40 ori
     misc_feature    4986..5582
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3604
                     /label=intron
     CDS             3651..4976
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3394
                     /label=hsa-mir-517a precursor
BASE COUNT     2177 a      2365 c      2488 g      2109 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg aggctgacgc 
     3121 aggagaatcg cttgaaccca ggaggcagaa gttgccatga gccgagatcc caccaccgca 
     3181 tccagcctgg gcggcagagt gagactccgt gtcaaaaaaa agaagatctc aggcagtgac 
     3241 cctctagatg gaagcactgt ctgttgtata aaagaaaaga tcgtgcatcc ctttagagtg 
     3301 ttactgtttg agacaagcaa cgttgaagat gctgctgatc ttggtaatac atttgcagag 
     3361 cgtgcttatc atcagacttg gatgatggtg gggtgctagc tcgagctttt ggagtacgtc 
     3421 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga 
     3481 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga 
     3541 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt 
     3601 tcaggtgtcg tgaaaactac ccctctagag tcgagctacc ggtcgccacc atggtgagca 
     3661 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg 
     3721 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 
     3781 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg 
     3841 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca 
     3901 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 
     3961 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca 
     4021 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga 
     4081 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg 
     4141 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga 
     4201 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt 
     4261 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg 
     4321 gccgccactc caccggcggc atggacgagc tgtacaagga cccaccggtc gccaccatga 
     4381 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca 
     4441 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc 
     4501 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 
     4561 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 
     4621 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 
     4681 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 
     4741 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 
     4801 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 
     4861 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 
     4921 tcgagtgccc gaaggaccgc gcgacctggt gcatgacccg caagcccggt gcctgagcgg 
     4981 ccgcgtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 
     5041 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 
     5101 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 
     5161 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 
     5221 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 
     5281 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 
     5341 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc 
     5401 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 
     5461 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 
     5521 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc 
     5581 tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 
     5641 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat 
     5701 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 
     5761 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 
     5821 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 
     5881 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 
     5941 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 
     6001 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     6061 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 
     6121 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 
     6181 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 
     6241 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc 
     6301 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt 
     6361 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga 
     6421 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc 
     6481 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 
     6541 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 
     6601 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 
     6661 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 
     6721 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 
     6781 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 
     6841 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 
     6901 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga 
     6961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 
     7021 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 
     7081 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 
     7141 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 
     7201 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 
     7261 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 
     7321 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 
     7381 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 
     7441 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 
     7501 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 
     7561 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 
     7621 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 
     7681 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 
     7741 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 
     7801 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 
     7861 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 
     7921 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 
     7981 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 
     8041 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 
     8101 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 
     8161 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 
     8221 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 
     8281 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 
     8341 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 
     8401 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 
     8461 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 
     8521 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 
     8581 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 
     8641 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 
     8701 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 
     8761 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 
     8821 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 
     8881 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 
     8941 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 
     9001 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 
     9061 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg 
     9121 gaacaaaagc tggagctgc 
//
